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Greater mobility and maturity also mean that your child is destined to meet more and more new people along his way These may be other families in the park or at the pool They may be cashiers or other shoppers at the grocery store, who now talk directly to him rather than just to you They may be the additional playmates who get added to his daycare group once he s graduated from the infant room As his circle of friends widens, he will more frequently encounter people who don t use signs Some signs, it s true, will always be useful For example, no matter what your age, an index finger brought to the lips continues to signal quiet and standing with your hands outstretched and palms up translates into the words I don t know or Where But most signs will inevitably

SITE-DIRECTED MUTAGENESIS can be used to change one or more base pairs in the DNA resulting in a change in the amino acid that appears in the protein produced from this DNA

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drop out in favor of the symbol system shared more widely vocal words All these new conversation partners, then, provide another strong incentive for your growing child to learn the words behind the signs

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This is a technique for amplifying a specific segment of DNA Oligonucleotide primers are synthesized that are complementary to one strand at the 5 end of your DNA and complementary to the opposite strand at the 3 end After the DNA is denatured and the oligonucleotide primers (in excess) are annealed, the DNA is elongated using DNA polymerase and deoxynucleotides A new double-stranded DNA molecule will be generated starting from each primer DNA sequences behind (to the 5 side of) the primer will not be replicated The DNA is then heated to denature it and reannealed to the primer again Another round of replication is performed This cycle is repeated over and over, with a twofold increase in the amount of DNA on each cycle Because two primers are used, only the sequence between the two primers will be amplified Since the cycle is carried out multiple times with a twofold increase in the amount of DNA each time, a geometric amplification results (10 cycles would result in a 210 increase in the DNA concentration) The limitation on the amount of DNA that is produced is the amount of primer and deoxynucleotides added The cleverness of this technique is extended by using a heat-stable DNA polymerase that is not inactivated by the temperatures needed to denature the DNA Multiple cycles can be performed simply by heating (denaturing) and cooling (renaturing and polymerizing) a tube containing the DNA, the primers, deoxynucleotide triphosphates, and the DNA polymerase Because of the extreme amount of amplification, PCR can be used to amplify sequences from very small amounts of DNA New restriction sites can be easily generated by including them in the 5 end of the oligonucleotide primer even though they are not present in the original DNA As long as the primer is still long enough to hybridize to the DNA through complementary sequences, the dangling 5 ends containing the restriction site sequence will be amplified in the next round PCR can also be used to remove inserts from vectors and to introduce site-specific mutants (Fig 6-7) By first making a DNA copy of an RNA molecule, one can also amplify RNA sequences Because reverse transcriptase (copies RNA to DNA) is used in the first step, this is called RT-PCR.

Getting older also means that your child will become increasingly skilled at using her body, particularly her hands, to explore and have fun in the world There are finger paints to spread around, crayons to color with, puzzles to put together, ladders to climb, and bikes to ride Each of these activities tends to keep hands pretty busy, making signs less and less convenient to produce Of course, we still take time out to wave bye-bye pretty much regardless of what we re doing But spoken words gain an edge over signs that they didn t have when your child was less dependent on her hands for a good time

Stan Gibilisco is one of McGraw-Hill s most diverse and bestselling authors Known for his clear, user-friendly, and entertaining writing style, Mr Gibilisco s depth of knowledge and ease of presentation make him an excellent choice for a book such as Astronomy Demystified His previous titles for McGraw-Hill include: The TAB Encyclopedia of Electronics for Technicians and Hobbyists, Teach Yourself Electricity and Electronics, and the Illustrated Dictionary of Electronics Booklist named his book, The McGraw-Hill Encyclopedia of Personal Computing one of the Best References of 1996

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