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contentious negotiations I can ever remember, each party having separate demands and reluctant to give up their independence But it was successful We achieved what was regarded as a monumental accomplishment a complex, tripartite agreement for a Globex joint venture (JV) On October 27, 1990, both memberships overwhelmingly (98 percent at the CME and 90 percent at the CBOT) approved the tripartite agreement A JV enterprise, The Globex Corporation, was created between the CME and the CBOT for the purpose of maintaining what was to become a jointly owned and operated global electronic trading system I was elected chairman of the Globex Corporation, and a star-studded cast was appointed as its board of directors: Jack Sandner, John Geldermann, Scott Gordon, Gordon McClendon, Tom Dittmer, Barry Lind, and William Shepard from the CME, and William O Connor, Dale Lorenzen, David Brennan, Charles Carey Jr.

Then the correlation matrix is denoted as (rho, the Greek letter for r): 2 2 2 1m 11 12 11 11 11 22 11 mm 2 2 2 2m 22 12 22 22 22 mm = 11 22 . . . .. . . . . . . . 2 2 2 2m mm 1m 11 mm 22 mm mm mm Consider again the standardized data matrix Z = V1/2 (X ). Then since each variable has been standardized, we have E(Z) = 0, where 0 denotes an n 1 vector 1 1 V1/2 = . Thus, for of zeros and Z has covariance matrix Cov(Z) = V1/2 the standardized data set, the covariance matrix and the correlation matrix are the same. The ith principal component of the standardized data matrix Z = [Z 1 , Z 2 , . . . , Z m ] is given by Yi = ei Z, where ei refers to the ith eigenvector (discussed below) and ei refers to the transpose of ei . The principal components are linear combinations Y1 , Y2 , . . . , Yk of the standardized variables in Z such that (1) the variances of the Yi are as large as possible, and (2) the Yi are uncorrelated. The rst principal component is the linear combination Y1 = e1 Z = e11 Z 1 + e12 Z 2 + + e1m Z m which has greater variability than any other possible linear combination of the Z variables. Thus: r The rst principal component is the linear combination Y1 = e Z, which max1 1

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8. ANALYTICAL ASSAYS 8.1. Mechanical Analysis Mechanical testing was performed with a servohydraulic Instron 8511 tension/ compression system with Fast-Track software. Fatigue analysis was performed on single ACL matrix cords. The data for a single cord were extrapolated to represent the six-cord ACL matrix because the cords were designed to be loaded in parallel. Single pull-to-failure testing was performed at a strain rate of 100%/s, and data were analyzed with Instron Series IX software. Fatigue testing to determine cycles to failure at ultimate tensile strength (UTS), 1680 N, and 1200 N (n = 5 for each load) was conducted with an H-sine wave function at 1 Hz generated by Wavemaker32 version 6.6 (Instron). Fatigue testing was performed in neutral phosphate-buffered saline (PBSA) at 23 C. Samples were stored after preparation for no more than 7 days at room temperature before testing. 8.2. Cell Viability Cell viability was determined by MTT staining. Seeded matrices were incubated in MTT solution (0.5 mg/ml MTT in cell culture medium) at 37 C under 5%CO2 for 2 h. The intense purple-colored formazan derivative formed via cell metabolism was eluted and dissolved in 0.04 M HCl in 95% isopropanol, and the absorbance was measured at 570 nm with a reference wavelength of 690 nm. Cell number was correlated to optical density (OD). 8.3. Morphology Phase-contrast light microscopy was used to observe the morphology of hBMSCs with a Zeiss Axiovert S100 light microscope. Digital image analysis incorporated a CCD color video camera (DXC-390, Sony), a frame grabber card (CG-7 RGB, Scion), and Scion Image software version 1.9.1.

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In this segment, I discuss the important development issues that surface as your application becomes more and more workable, and the time to package and distribute your work grows nearer.

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